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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Rapid Commun Mass Spectrom. 2020 Feb 11;34(Suppl 1):e8552. doi: 10.1002/rcm.8552

Figure 1. Western Blot Reveals Increased HKa.

Figure 1.

A. Schematic comparing the structure of LK to HK. LK and HK share identical D1 to D4. LK contains an alternate D5 and lacks D6. 120 kDa HK contains six functional domains. D4/BK release generates HKa, composed of the 65 kDa HC connected by disulfide bond to the 56 kDa LC. Further proteolysis produces a 45 kDa LC and D5 peptide release. B. Representative blot showing four bands (120, 100, 65, 56 kDa) derived from HK. C–F. Bar graphs showing mean densitometry values with SEM. T2 and T3 values are relative to T1, which is set equal to one. Friedman’s test for repeated measurements of non-parametric data was used to obtain p-values. C. 120 kDa band representing intact HK significantly decreases during T1 to T2 and T1 to T3. D. 100 kDa band increases T1 to T2 and T1 to T3. E. 65 kDa band representing the HC increases significantly during pregnancy. F. 56 kDa band representing the LC increases significantly during pregnancy. * p < 0.05; ** p < 0.01; **** p < .0001; ns not significant.