Table 1.

Table of all HK derived peptides presenting ten or more complete data sets.

							Multiple Comparison			Median (log2)
Mass	n	Start	End	Trend	Normal	P-value	T1 vs T2	T1 vs T3	T2 vs T3	T1	T2	T3	Prev Rep Seq	Domain	Sequence
1233.626	48	381	391	+	N	0.0005	ns	***	ns	17.77	18.17	18.41		D4	RPPGFSPFRSS
1347.655	25	428	437	-	Y	.0004	*	****	ns	20.38	19.32	18.82		D5	RHDWGHEKQR
1357.758	47	185	196	0	Y	ns	ns	ns	ns	18.1	18.14	17.74		D2	AQRQVVAGLNFR
1389.727	49	381	392	-	N	0.0004	*	***	ns	20.84	20.49	20.07		D4	RPPGFSPFRSSR
1510.718	21	465	477	+	N	0.0119	*	ns	ns	17.66	19.16	18.93		D5	HEQQHGLGHGHKF
1599.74	25	459	473	0	Y	ns	ns	ns	ns	20.35	20.79	21.03		D5	HGLGHGHEQQHGLGH
1704.798	41	463	477	+	Y	0.001	**	**	ns	22.02	22.91	23.23		D5	HGHEQQHGLGHGHKF
1761.82	25	462	477	0	Y	ns	ns	ns	ns	20	20.32	20.8		D5	GHGHEQQHGLGHGHKF
1793.821	36	459	475	0	Y	ns	ns	ns	ns	22.28	22.86	22.56		D5	HGLGHGHEQQHGLGHGH
1850.842	17	458	475	0	Y	ns	ns	ns	ns	21.06	21.82	21.55	X	D5	GHGLGHGHEQQHGLGHGH
1874.904	39	461	477	+	Y	0.003	**	ns	ns	18.63	19.75	19.72		D5	LGHGHEQQHGLGHGHKF
1931.925	45	460	477	+	N	0.0003	*	***	ns	21.13	22.45	22.72		D5	GLGHGHEQQHGLGHGHKF
2006.943	21	457	475	+	N	0.0006	*	***	ns	19.58	21.55	21.56	X	D5	RGHGLGHGHEQQHGLGHGH
2068.984	50	459	477	+	N	<.0001	***	****	ns	24.24	25.67	25.79		D5	HGLGHGHEQQHGLGHGHKF
2126.006	49	458	477	+	N	<.0001	***	****	ns	25.16	26.24	26.26	X, A	D5	GHGLGHGHEQQHGLGHGHKF
2254.101	50	458	478	+	N	0.0002	***	***	ns	24.15	25.14	25.48	X, A	D5	GHGLGHGHEQQHGLGHGHKFK
2282.107	43	457	477	+	N	<.0001	***	****	ns	19.97	21.64	22.51	X, A	D5	RGHGLGHGHEQQHGLGHGHKF
2286.089	30	478	497	+	N	0.0177	ns	*	ns	17.68	18.46	18.4		D5	KLDDDLEHQGGHVLDHGHKH
2367.185	46	458	479	+	Y	<.0001	****	****	ns	20.78	22.32	22.68	X, A	D5	GHGLGHGHEQQHGLGHGHKFKL
2403.158	26	460	481	0	Y	ns	ns	ns	ns	18.86	19.03	19.09		D5	GLGHGHEQQHGLGHGHKFKLDD
2410.165	19	456	477	+	Y	0.016	ns	*	ns	18.98	20.22	20.87	X, A	D5	QRGHGLGHGHEQQHGLGHGHKF
2482.212	34	458	480	+	Y	<.0001	**	****	**	17.76	19.19	19.28	X, A	D5	GHGLGHGHEQQHGLGHGHKFKLD
2547.224	21	455	477	+	Y	0.004	*	*	ns	17.53	18.73	19.06	X, A	D5	HQRGHGLGHGHEQQHGLGHGHKF
2597.238	48	458	481	+	Y	0.0005	*	**	ns	21.31	22.11	22.09	X, A	D5	GHGLGHGHEQQHGLGHGHKFKLDD
2698.311	19	475	497	+	N	0.0294	*	ns	ns	18.5	19.67	19.23		D5	HKFKLDDDLEHQGGHVLDHGHKH
2785.363	19	438	462	+	Y	0.0403	ns	ns	ns	18.18	18.88	19.67		D5	KHNLGHGHKHERDQGHGHQRGHGLG
2892.392	25	473	497	+	N	0.0027	*	**	ns	18.32	19.76	19.99		D5	HGHKFKLDDDLEHQGGHVLDHGHKH
2996.425	28	478	504	0	Y	ns	ns	ns	ns	19.27	19.6	19.57		D5	KLDDDLEHQGGHVLDHGHKHKHGHGHG
2996.425	25	479	505	+	Y	0.006	*	*	ns	18.46	19.87	19.34		D5	LDDDLEHQGGHVLDHGHKHKHGHGHGK
3020.4	22	480	506	0	N	ns	ns	ns	ns	20.48	22.03	21.31		D5	DDDLEHQGGHVLDHGHKHKHGHGHGKH
3041.412	45	450	477	+	N	<.0001	**	****	ns	20.59	21.74	22.06	X, A	D5	DQGHGHQRGHGLGHGHEQQHGLGHGHKF
3133.484	46	479	506	+	N	<.0001	**	****	ns	19.9	20.9	21.75		D5	LDDDLEHQGGHVLDHGHKHKHGHGHGKH
3197.513	34	449	477	+	Y	0.0004	**	**	ns	20.87	22.54	22.39	X, A	D5	RDQGHGHQRGHGLGHGHEQQHGLGHGHKF
3217.6	13	482	510	0	Y	ns	ns	ns	ns	21.25	21.14	20.52		D5	DLEHQGGHVLDHGHKHKHGHGHGKHKNKG
3219.51	17	458	486	0	Y	ns	ns	ns	ns	17.4	17.38	17.6	X, A	D5	GHGLGHGHEQQHGLGHGHKFKLDDDLEHQ
3261.579	34	478	506	+	Y	0.002	**	**	ns	19.13	20.63	20.66		D5	KLDDDLEHQGGHVLDHGHKHKHGHGHGKH
3262.538	11	480	508	+	Y	0.01	ns	*	ns	21.49	23.26	22.98		D5	DDDLEHQGGHVLDHGHKHKHGHGHGKHKN
3276.531	20	458	487	0	Y	ns	ns	ns	ns	17.54	17.8	18.01	X, A	D5	GHGLGHGHEQQHGLGHGHKFKLDDDLEHQG
3326.555	25	448	477	0	Y	ns	ns	ns	ns	18.22	18.84	19.38	X, A	D5	ERDQGHGHQRGHGLGHGHEQQHGLGHGHKF
3375.622	39	479	508	+	Y	.002	**	*	ns	21.11	21.8	22		D5	LDDDLEHQGGHVLDHGHKHKHGHGHGKHKN
3381.597	43	429	456	0	N	ns	ns	ns	ns	25.62	25.59	25.38		D5	HDWGHEKQRKHNLGHGHKHERDQGHGHQ
3408.647	14	477	506	0	N	ns	ns	ns	ns	17.44	18.63	18.64		D5	FKLDDDLEHQGGHVLDHGHKHKHGHGHGKH
3447.654	28	480	510	+	Y	0.023	**	ns	ns	21.87	24.09	24.24		D5	DDDLEHQGGHVLDHGHKHKHGHGHGKHKNKG
3463.614	37	447	477	+	Y	0.009	*	*	ns	20.92	22.14	21.97	X, A	D5	HERDQGHGHQRGHGLGHGHEQQHGLGHGHKF
3503.717	42	478	508	0	Y	ns	ns	ns	ns	20.44	21.11	20.93		D5	KLDDDLEHQGGHVLDHGHKHKHGHGHGKHKN
3537.699	49	428	456	-	N	0.0026	ns	**	ns	28.66	28.3	27.63		D5	RHDWGHEKQRKHNLGHGHKHERDQGHGHQ
3560.738	48	479	510	+	Y	<.0001	****	****	ns	22.36	24.11	24.39		D5	LDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKG
3638.721	22	438	469	0	Y	ns	ns	ns	ns	20.82	18.69	20.84		D5	KHNLGHGHKHERDQGHGHQRGHGLGHGHEQQH
3650.785	15	477	508	0	N	ns	ns	ns	ns	18.43	19.09	19.46		D5	FKLDDDLEHQGGHVLDHGHKHKHGHGHGKHKN
3688.833	45	478	510	+	Y	<.0001	****	***	ns	20.75	22.03	22.37		D5	KLDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKG
3688.833	37	479	511	+	Y	<.0001	****	***	ns	19.55	21.56	21.54		D5	LDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKGK
3693.8	12	428	457	-	Y	0.027	*	ns	ns	24.49	23.07	22.26		D5	RHDWGHEKQRKHNLGHGHKHERDQGHGHQR
3816.928	23	479	512	+	N	0.0005	**	***	ns	18.64	20.06	20.02		D5	LDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKGKK
3835.902	33	477	510	+	Y	<.0001	**	****	ns	18.32	20.06	20.55		D5	FKLDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKG
3865.848	27	438	472	0	Y	ns	ns	ns	ns	21.29	22.15	21.85		D5	KHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLG
3987.993	19	479	514	+	Y	<.0001	***	**	ns	19.06	20.84	20.64	^	D5	LDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKGKKNG
4002.907	35	438	473	0	N	ns	ns	ns	ns	22.5	23.27	23.18	X	D5	KHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGH
4196.987	45	438	475	+	N	<.0001	**	****	ns	24.98	26.61	26.37	X	D5	KHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGH
4344.056	23	439	477	+	N	0.0006	ns	***	ns	22.55	23.91	24.49	X, A	D5	HNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKF
4394.084	48	458	497	+	Y	<.0001	****	****	*	21.22	22.61	23.04	X, $, A	D5	GHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKH
4472.151	41	438	477	+	N	0.0001	ns	****	ns	26.55	28.04	28.46	X, A	D5	HNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKFK
4472.151	48	439	478	+	N	0.0011	ns	***	ns	26.2	27.66	27.94	X, A	D5	KHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKF
4522.179	38	458	498	+	N	<.0001	**	****	ns	19.02	20.46	20.88	X, $, A	D5	GHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHK
4600.246	36	438	478	0	N	ns	ns	ns	ns	24.01	24.07	23.5	X, A	D5	KHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKFK
4713.33	30	438	479	0	Y	ns	ns	ns	ns	21.4	22.42	22.6	X, A	D5	KHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKFKL
4943.384	21	438	481	0	N	ns	ns	ns	ns	20.32	20.46	20.64	X, A	D5	KHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKFKLDD
5104.42	35	458	504	+	Y	0.028	ns	***	*	19.87	20.29	20.84	X, $, A	D5	GHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHG
5232.515	23	458	505	0	Y	ns	ns	ns	ns	19.9	20.21	20.65	X, $, A	D5	GHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHGK
5369.574	10	458	506	0	Y	ns	ns	ns	ns	19.42	19.78	20.16	X, $, A	D5	GHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHGKH
5611.712	48	458	508	0	N	ns	ns	ns	ns	20.94	21.52	21.64	X, $, A	D5	GHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHGKHKN
5796.828	36	458	510	+	Y	<.0001	***	****	ns	21.61	23.17	23.68	X, $, A	D5	GHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKG
5924.923	26	458	511	+	N	0.0002	ns	****	ns	19.78	20.67	21.4	X, $, A	D5	GHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKGK
6740.229	31	438	497	+	Y	<.0001	***	****	**	20.58	22.04	22.83	X, $, A	D5	KHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKH

n = number of complete data sets. “Start” and “End” are the positions of the starting and ending amino acids, numbering corresponds to UniProt sequence. “Trend” indicates a peptide’s change in abundance during pregnancy, where “+” indicates an increase, “-” indicates a decrease, and “0” indicates no significant trend. Normality was tested using the D’Agostino-Pearson omnibus normality test; the result is indicated in the “Normal” column, where “Y” = normal and “N” = not normal. For normally distributed data, p-values were determined by repeated-measurements one-way ANOVA and Tukey’s multiple comparison. For non-normally distributed data, p-values were determined by Friedman test and Dunn’s multiple comparison. p-values derived from multiple comparison tests are represented by stars according to Prism 6’s output, where * is p < 0.05; ** is p < 0.01; *** is p < .001; **** is p < .0001; and ns is not significant. In the “Prev Rep Seq” column, “X” indicates a peptide that contains the previously reported Gly58-His473³³ sequence, “$” indicates His459-Asp492³³, A indicates Gly458-Phe477²⁷, and “^” indicates Lys498-Asn513³⁰.