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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: FASEB J. 2019 Dec 23;34(2):2392–2407. doi: 10.1096/fj.201902227R

Figure 2. Overexpression of miR182 promotes TNF-induced osteoclastogenesis.

Figure 2.

A. Osteoclast differentiation using BMMs derived from Ctrl and Mir182mTg mice stimulated with or without TNFα (40 ng/mL) for three days. TRAP staining (left panel) was performed and the area of TRAP-positive MNCs (≥ 3 nuclei/cell) per well relative to the control was calculated (right panel). TRAP-positive cells appear red in the photographs. Scale bar: 200 μm. B. Quantitative real-time PCR (qPCR) analysis of mRNA expression of Nfatc1 (encoding NFATc1), Prdm1 (encoding Blimp1), Ctsk (encoding cathepsin K), Calcr (encoding calcitonin receptor) and Acp5 (encoding TRAP) during osteoclastogenesis using BMMs from the Ctrl and Mir182mTg mice treated with or without TNFα for two days. C. Immunoblot analysis of the expression of NFATc1, Blimp1 and IRF8 induced by TNFα at the indicated times. GAPDH was used as a loading control. Data are mean ± SEM. **p < 0.01.