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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: FASEB J. 2019 Dec 12;34(2):2301–2311. doi: 10.1096/fj.201902278RR

Figure 1.

Figure 1

Mdm2 expression is regulated by iron. (A) HEPG2 cells were mock-treated or treated with various amounts of DFO or FAC, followed by western blot analysis to examine the levels of IRP2, Mdm2 and actin proteins. (B-C) MCF7 (B) and HCT116 (C) cells were mock-treated or treated with DFO (0-300 μM, 24h) for 24 hours and the levels of IRP2, TFR, Mdm2, p53, and actin proteins were examined by western blot analysis. (D-E) HCT116-p53-KO (D) and Mia-PaCa2 (E) cells were mock-treated or treated with DFO (0-300 μM, 24h) for 24 hours and the levels of IRP2, TFR, Mdm2, and actin proteins were examined by western blot analysis.