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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: FASEB J. 2019 Dec 12;34(2):2301–2311. doi: 10.1096/fj.201902278RR

Figure 2.

Figure 2

IRP2, but not IRP1, is required for iron-mediated Mdm2 expression. (A-B) H1299 cells were uninduced or induced to express HA-tagged IRP1 (A) or IRP2 (B) for 24 hours, the levels of IRP1 (A), IRP2 (B), Mdm2, and actin proteins were examined by western blot analysis. (C) The level of IRP1, Mdm2, and GAPDH transcripts was measured in Mia-PaCa2 cells transfected with a scrambled or IRP1 siRNA for 3 days. (D) HCT116 and MCF7 cells were transfected with a scrambled or IRP2 siRNA for 3 days, and the levels of IRP2, Mdm2 and vinculin were examined by western blot analysis. (E-F) Isogenic control and IRP2-KO p53−/−HCT116 (D) and Mia-PaCa2 (E) cells were used to examine the levels of IRP2, Mdm2, vinculin (D) and actin (E) proteins by western blot analysis.