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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: FASEB J. 2019 Dec 23;34(2):2326–2343. doi: 10.1096/fj.201902542R

Figure 2. N-glycans recognized by PHA-E and GNA expressed on human CD11b.

Figure 2.

(A) Purified CD11b/CD18 was immunoblotted using biotinylated PHA-E, GNA lectins or antibodies against sLex and Lex. (B) Immunoblots showing the CD11b/CD18 glycans recognized by PHA-E and GNA are N-glycan linked. (C) Total cell lysates from human PMN were immunoblotted with antibodies against CD11b and CD18 or biotinylated PHA-E and GNA lectins. (D) Changes in surface expression of CD11b/CD18, CD11a/CD18 and glycans recognized by PHA-E and GNA on human PMNs were analyzed by flow cytometry in non-stimulated (Ntx) PMNs and PMNs exposed to 100nM FMLF or 10nM LTB4 using FITC conjugated mAbs or lectins. Data are mean fluorescent intensities ± SEM (n=5). *, p<0.05, **, p<0.01, ***, p<0.001.