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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: FASEB J. 2019 Dec 16;34(2):2126–2146. doi: 10.1096/fj.201901301R

Figure 10. JNK2 deficiency enhances contractile proteins expression.

Figure 10.

A: Immunofluorescence analysis of smooth muscle marker proteins expression in WT and JNK2 KO mice. Bladder sections prepared from WT and JNK2 KO mice were stained with anti-SMA or anti-SM22 or SMHC antibody followed by Cy3- and Alexa Fluor 488 conjugated secondary antibodies. Representative confocal images are shown. Scale bars = 50 μm. B: Quantification of confocal images. Data are expressed as means ± SEM. n = 5 mice in each group. **, P < 0.01 versus WT mice. C: Total proteins of BSM strips from WT and JNK2 KO mice were subjected to immunoblot analysis and the expression levels of smooth muscle marker proteins were determined. Representative immunoblot is shown. GAPDH was used as a loading control. D: Quantification of immunoblot.