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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: FASEB J. 2019 Dec 16;34(2):2126–2146. doi: 10.1096/fj.201901301R

Figure 7. Effect of vimentin overexpression on smooth muscle marker proteins expression in murine BSM.

Figure 7.

A: Murine BSM strips devoid of urothelium and submucosa were transduced with an adenovirus encoding GFP, and vimentin for 48 h and the expression levels of SMA, and SM22 were determined by immunoblot analysis. GAPDH was used as a loading control. B: Quantification of immunoblot data. C-D: Sections prepared from murine BSM strips overexpressing vimentin and GFP proteins were stained with anti-SMA, or anti- SM22, or anti-SMHC or Alexa Fluor 488 labelled GFP antibody, followed by Cy3 and Cy5 conjugated secondary antibodies. Representative confocal images are shown in C and D: Scale bars = 50 μm. E: Quantification of confocal images data. Data are expressed as means ± SD, n = 5 mice in each group. NS indicates non significant.