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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: FASEB J. 2019 Dec 16;34(2):2126–2146. doi: 10.1096/fj.201901301R

Figure 8. Desmin and vimentin interact with JNK2 and enhance the phospho JNK level following the IF protein overexpression in murine BSM.

Figure 8.

A-B: Murine BSM strips devoid of urothelium and submucosa were transduced with an adenovirus encoding GFP, desmin and vimentin for 48 h and the total proteins from GFP, desmin and vimentin overexpressing murine BSM strips were immunoprecipitated with JNK2 antibody. The resulting immunoprecipitate was separated on a SDS-PAGE and subsequently probed with either anti-desmin and anti-JNK2 antibodies (A) or anti-vimentin and anti JNK2 antibodies (B). The input served as a loading control in both (A) and (B). C-F: Increased levels of phospho JNK following desmin and vimentin overexpression. Total proteins extracted from GFP, desmin and vimentin overexpressing murine BSM strips were subjected to immunoblot analysis using phosphorylated JNK and total JNK2 antibodies. GAPDH was used as a loading control. D & F: Quantification of immunoblots (C & E). Data are expressed as means ± SD, n = 5 mice in each group. **, P < 0.01 versus GFP expressing murine BSM strips.