Figure 5.

FcεR1 expression on Ly6G⁺ neutrophils in Ang-II perfusion-induced AAA lesions. Immunofluorescent staining of Ly6G (green) and FcεR1 (red) double positive neutrophils in the adventitia (Adv.) (A), media (Med.) next to the lumen (L) (B), and peri-aorta (Peri-Ao.) inflammatory cell clusters (C) in AAA lesions from Apoe^−/− mice. D. Ly6G (green) and FcεR1 (red) double positive neutrophils the adventitia (Adv.) in AAA lesions from the Apoe^−/−Ige^−/− mice. E. AAA lesions from Apoe^−/−Fcer1a^−/− mice were used as negative control to detect FcεR1 expression (red) on Ly6G neutrophils (green). Scale: 200 μm; insert: 70 μm. F. FACS analysis of Ly6G⁺FcεR1⁺ neutrophils in AAA lesions from Apoe^−/− and Apoe^−/−Fcer1a^−/− mice (negative control).