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. Author manuscript; available in PMC: 2020 Jul 20.
Published in final edited form as: Nat Med. 2020 Jan 20;26(2):244–251. doi: 10.1038/s41591-019-0730-x

Extended Data Fig. 4: Experimental manipulation of NEK10 activity alters ciliated cell morphology.

Extended Data Fig. 4:

a, gating strategy for imaging flow cytometry analysis of MCCs. b, representative images and masking data of cells in (a), demonstrating ability to generate single NEK10:eGFP+ ciliated cells for analysis. c, confocal maximum intensity projections of mature ALI edited with the indicated sgRNAs after IF against Ac-α-tubulin, scale bars 25μm, representative of 2 independent ALI differentiations. d, confocal maximum intensity projections of mature ALI transduced with the indicated cDNAs after IF against Ac-α-tubulin, scale bars 25μm, representative of 2 independent ALI differentiations. e, H&E stained mature ALI samples of the indicated genotypes after sectioning orthogonal to the epithelial surface, representative of 3 independent ALI differentiations.