Extended Data Fig. 5: Structural and proteomic abnormalities in NEK10-deficient airway epithelium.

a, SEM of mature ALI edited with the indicated sgRNAs, scale bars 100μm (upper panels) and 1μm (lower panels), representative of 2 independent ALI differentiations. b, immunoblotting against the indicated proteins from lysates generated from purified cilia (lanes 2, 4) or remaining de-ciliated mature ALI (lanes 1, 3), representative of 2 experiments. c, cumulative distribution of phosphopeptides by log₂ fold change between indicated conditions, solid (sgNEK10b) and dashed (sgNEK10c) red lines illustrate population of depleted phosphopeptides upon NEK10 deletion. d, table of gene ontology classes enriched among genes (n=395) whose peptides are depleted >1.5 fold (log₂) after targeting with sgNEK10b, enrichment level, p-values, and false discovery rates (FDR) indicated. e, cumulative distribution of phosphopeptides by log₂ fold change, previously validated PCD in red and all other detected proteins in black, as in (4e). f, cumulative distribution of phosphopeptides by log₂ fold change, previously validated non-PCD ciliopathy loci in red and all other detected proteins in black, as in (4e).