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. 2020 Feb 11;11(1):e03253-19. doi: 10.1128/mBio.03253-19

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Copyright © 2020 Abraham et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Replication of SINV WT and nsP3^MD mutants in mouse neuronal NSC34 cells. NSC34 cells were infected with SINV WT (TE strain) and nsP3 MD mutants N24A, G32A, G32E, G32S, Y114A, and TM (G32E/I113R/Y114N) at an MOI of 10. (A) Virus production was measured by plaque formation in Vero cells. The data are presented as means ± the SD obtained from three independent experiments *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (TE versus nsP3 MD mutants N24A, G32A, G32E, Y114A, and TM). (B) Cell viability after infection was determined by trypan blue exclusion. The data are presented as means ± the SD obtained from three independent experiments of the numbers of viable cells compared to day 0 expressed as a percentage. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (G32E versus TE and nsP3 MD mutants N24A, G32A, G32S, Y114A, and TM). Significance was determined by two-way ANOVA with Tukey’s multiple-comparison test.