Fig. 6.

Periodontal inflammation can promote head and neck metastasis. a Timeline of the late metastasis model, PI was induced with LPS for 3 weeks then mice were injected in the mammary fat pad with 4T1 cells, the primary tumor was let to grow until it reached 100 mm³ in size, surgery was accomplished to extract this tumor and animals were let to grow metastasis and euthanized at 10.5 weeks. b Metastasis were observed and counted in lungs and lymph node in the individual animal groups in the presence or absence of anakinra. (n ≥ 3; ANOVA). c IHC staining of Pan-cytokeratin and Gr1 from the lymph node and the cervical tissue of late metastasis model. (n ≥ 3; Ank anakinra, ANOVA, *P < 0.05; **P < 0.01; ****P < 0.0001)