FIGURE 7.

MTB H37Rv induced less apoptosis than other mycobacterial species. (A) Quantitation of THP-1 cell apoptosis by TUNEL staining 24 h after infection with the individual mycobacterial species. (B) Annexin-V expression was quantified in THP-1 macrophages infected overnight with various mycobacterial species. Data shown are the mean ± SEM of three independent experiments. (C) Cytochrome C, Bax, and Bak expression was determined by immunoblotting of the lysates of THP-1 cells infected with various NTM species and MTB H37Rv. Immunoblots shown are representative of three independent experiments. Unless otherwise indicated, *p < 0.05, **p < 0.01 are compared to unstimulated control cells (open bars). Multiplicity-of-infection of 10 mycobacteria:1 macrophage.