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CLSM analysis of biofilm of MRSA ATCC 43300 preventively incubated with either Sb or PYO. The viability of the cells attached on the surface was evaluated after 24 h incubation staining with green fluorescent labeled SYTO9 (488/500–540 nm) for alive bacteria and with red fluorescent propidium iodide (PI) (561/600–650 nm) for dead bacteria. Images are merged from the two channels. Upper and lower panels represent xy- and z-plans, respectively. Scale bar: 25 μm.
