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. 2020 Feb 13;9(2):18. doi: 10.1038/s41389-020-0201-8

Fig. 1. PERK-mediated secretion of proteins under hypoxia.

Fig. 1

a Total PERK, eIF2α and P-eIF2α expression in HEK293, LN308 and LN229 cell lines under normoxia or hypoxia. b Active form of ATF6α (50 kDa band) in LN308, under hypoxia for 24, 48 and 72 h. EEF2 was used as a loading control. c Relative mRNA levels of XBP1s as determined by qRT-PCR in LN308 and LN229 cell lines under hypoxia (48 h). NDRG1 was taken as a positive control for hypoxia induction. EEF2 was used as housekeeping gene. Data are normalized to the respective normoxic conditions and are represented as the mean of three independent experiments ± SEM (T test: **p value < 0.01). N normoxia, H hypoxia. d Total XBP1s mRNA transcripts in HEK293 and LN308 cells treated with hypoxia for 24, 48, 72 h. β-actin was used as a housekeeping gene. e Total P-IRE1α species immunoprecipitated using P-IRE1α antibody from HEK293 and LN308 cells treated with hypoxia for 48 h. f Volcano plot representing the regulated secretory proteins from LN308 glioblastoma cells under hypoxic conditions for 72 h without (left) and with PERK inhibitor (GSK2606414; right). The data are represented as the mean of three independent replicates. The significant p value cut-off was set at 0.05.