Table 3.
Physio-chemical characterization of Mfps (Generated in Expasy protparam) with signal peptide prediction (Generated in Phobius and SignaIP 5.0).
| Sl. No | Bivalve | Variant | Signal Peptide Prediction | pI | Mw (Dalton) | II | AI | EC | GRAVY | Half-life (in Hours) – in vivo | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SignaIP 5.0 | Phobius | Yeast | E. coli | |||||||||
| 1 | Mytilus californianus | Mcfp1 v1 | 1–24 | 1–20 | 10.04 | 85024.22 | 41.20 | 26.57 | 204255 | −1.357 | 20 | 10 |
| 2 | Mcfp1 v2 | 1–24 | 1–20 | 10.04 | 78048.08 | 40.93 | 28.07 | 186375 | −1.329 | 20 | 10 | |
| 3 | Mcfp2 | 1–27 | 1–17 | 9.04 | 46753.52 | 14.42 | 27.80 | 58545 | −0.890 | 20 | 10 | |
| 4 | Mcfp3 v1 | 1–24 | 1–22 | 10.09 | 7548.70 | 25.90 | 61.97 | 31400 | −0.658 | 20 | 10 | |
| 5 | Mcfp3 v2 | 1–26 | 1–19 | 10.05 | 7791.92 | 23.61 | 61.62 | 36900 | −0.629 | 20 | 10 | |
| 6 | Mcfp3 v3 | 1–24 | 1–22 | 8.80 | 8836.73 | 13.29 | 55.00 | 42860 | −0.505 | 20 | 10 | |
| 7 | Mcfp3 v4 | 1–24 | 1–22 | 9.07 | 8973.87 | 19.12 | 48.72 | 44350 | −0.649 | 20 | 10 | |
| 8 | Mcfp3 v5 | 1–24 | 1–22 | 9.07 | 9000.90 | 13.12 | 48.72 | 44350 | −0.683 | 20 | 10 | |
| 9 | Mcfp3 v6 | 1–24 | 1–22 | 9.70 | 9120.28 | 32.33 | 48.72 | 46870 | −0.791 | 20 | 10 | |
| 10 | Mcfp3 v7 | 1–24 | 1–22 | 7.88 | 8623.45 | 11.60 | 50.67 | 42860 | −0.631 | 20 | 10 | |
| 11 | Mcfp3 v8 | 1–24 | 1–22 | 8.86 | 7920.73 | 12.00 | 55.07 | 39880 | −0.626 | 20 | 10 | |
| 12 | Mcfp3 v9 | 1–24 | 1–22 | 7.94 | 7799.59 | 8.98 | 60.72 | 38390 | -0.501 | 20 | 10 | |
| 13 | Mcfp3 v10 | 1–24 | 1–22 | 7.94 | 7786.59 | 8.17 | 60.72 | 38390 | −0.461 | 20 | 10 | |
| 14 | Mcfp3 v11 | 1–24 | 1–22 | 7.94 | 7790.59 | 12.19 | 56.52 | 38390 | −0.439 | 20 | 10 | |
| 15 | Mcfp4 v1 | 1–26 | 1–19 | 10.20 | 90254.35 | 43.14 | 87.04 | 30955 | −0.835 | 20 | 10 | |
| 16 | Mcfp4 v2 | 1–26 | 1–19 | 10.48 | 95385.30 | 42.38 | 87.65 | 30955 | −0.853 | 20 | 10 | |
| 17 | Mcfp5 | 1–25 | 1–18 | 9.77 | 10928.51 | 20.17 | 47.71 | 29925 | −0.847 | 20 | 10 | |
| 18 | Mcfp6 v1 | 1–24 | 1–22 | 8.85 | 13928.70 | 45.50 | 41.07 | 33530 | −0.550 | 20 | 10 | |
| 19 | Mcfp6 v2 | 1–25 | 1–22 | 9.24 | 13911.86 | 57.41 | 42.73 | 30550 | −0.544 | 20 | 10 | |
| 20 | Mcfp6 v3 | 1–24 | 1–22 | 9.24 | 13824.73 | 55.42 | 42.73 | 30550 | −0.551 | 20 | 10 | |
| 21 | Mcfp7 v1 | 1–30 | 1–24 | 10.41 | 6333.36 | 23.99 | 71.02 | 8940 | −0.163 | 20 | 10 | |
| 22 | Mcfp7 v2 | 1–30 | 1–25 | 10.17 | 6688.79 | 7.99 | 62.92 | 8940 | −0.245 | 20 | 10 | |
| 23 | Mcfp8 | 1–26 | 1–26 | 10.00 | 7281.66 | −7.47 | 70.58 | 16390 | −0.122 | 20 | 10 | |
| 24 | Mcfp9 v1 | 1–21 | 1–19 | 9.74 | 13430.87 | 22.53 | 69.84 | 17420 | −0.330 | 20 | 10 | |
| 25 | Mcfp9 v2 | 1–22 | 1–19 | 9.74 | 13312.69 | 29.75 | 66.08 | 17420 | −0.466 | 20 | 10 | |
| 26 | Mcfp10 | 1–24 | 1–16 | 8.59 | 6316.67 | 29.41 | 68.68 | 82795 | −0.285 | 20 | 10 | |
| 27 | Mcfp11 | 1–30 | 1–20 | 9.89 | 58635.75 | 36.53 | 55.46 | 108585 | −0.914 | 20 | 10 | |
| 28 | Mcfp12 | 1–25 | 1–21 | 9.92 | 82004.53 | 41.55 | 55.55 | 144845 | −0.831 | 20 | 10 | |
| 29 | Mcfp13 | 1–30 | 1–22 | 10.35 | 15181.82 | 44.67 | 72.20 | 35425 | −0.482 | 20 | 10 | |
| 30 | Mcfp14 | 1–23 | 1–23 | 8.65 | 13006.00 | 41.16 | 75.50 | 10720 | −0.071 | 20 | 10 | |
| 31 | Mcfp15 | 1–21 | 1–18 | 9.52 | 2876.43 | 42.30 | 50.12 | 47635 | −0.794 | 20 | 10 | |
| 32 | Mcfp16 | 1–25 | 1–19 | 9.33 | 10630.48 | 33.36 | 50.74 | 3730 | −0.666 | 20 | 10 | |
| 33 | Mcfp17 | 1–27 | 1–27 | 9.21 | 23576.23 | 36.72 | 65.22 | 70205 | −0.446 | 20 | 10 | |
| 34 | Mcfp18 | 1–22 | 1–20 | 8.81 | 7925.25 | 47.24 | 63.75 | 9565 | −0.026 | 20 | 10 | |
| 35 | Atrina pectinate | Apfp1 | 1–25 | 1–18 | 9.47 | 40382.27 | 33.74 | 57.76 | 121185 | −0.662 | 20 | 10 |
| 36 | Dreissena polymorpha | Dpfp1 | 1–30 | 1–19 | 5.24 | 49361.70 | 53.65 | 31.40 | 122860 | −1.331 | 20 | 10 |
| 37 | Mytilus edulis | Mefp1 | 1–30 | 1–20 | 9.99 | 6467.22 | 36.51 | 26.02 | 152105 | −1.280 | 20 | 10 |
| 38 | Mefp2 | 1–23 | 1–17 | 9.14 | 54459.22 | 18.05 | 26.47 | 59630 | −0.896 | 20 | 10 | |
| 39 | Mytilus galloprovincialis | Mgfp1 | 1–34 | 1–34 | 9.99 | 85791.18 | 44.31 | 20.80 | 213195 | −1.353 | 20 | 10 |
| 40 | Mgfp3 v1 | 1–25 | 1–24 | 10.24 | 8003.02 | 48.41 | 55.71 | 31400 | −0.801 | 20 | 10 | |
| 41 | Mgfp3 v2 | 1–25 | 1–24 | 10.32 | 843.79 | 54.86 | 49.35 | 38390 | −0.908 | 20 | 10 | |
| 42 | Mytilus unguiculatus | Mufp2 | 1–26 | 1–17 | 9.23 | 31206.12 | 16.16 | 34.30 | 42145 | −0.842 | 20 | 10 |
| 43 | Mufp3 | 1–27 | 1–27 | 9.13 | 8769.96 | 6.05 | 63.72 | 42860 | −0.350 | 20 | 10 | |
| 44 | Mufp3 v1 | 1–26 | 1–19 | 9.13 | 8715.64 | 15.14 | 66.28 | 38850 | −0.426 | 20 | 10 | |
| 45 | Mufp3 v2 | 1–25 | 1–19 | 8.64 | 8701.63 | 13.24 | 66.28 | 44350 | −0.383 | 20 | 10 | |
| 46 | Mufp3 v3 | 1–25 | 1–19 | 10.33 | 7696.70 | 42.06 | 60.61 | 29910 | −0.889 | 20 | 10 | |
| 47 | Mufp3 v4 | 1–25 | 1–19 | 8.80 | 8729.62 | 18.18 | 61.28 | 44350 | −0.449 | 20 | 10 | |
| 48 | Mufp3 v5 | 1–25 | 1–19 | 8.66 | 8685.63 | 14.33 | 66.28 | 42860 | −0.331 | 20 | 10 | |
| 49 | Mufp3 v6 | 1–25 | 1–19 | 7.90 | 8689.55 | 22.48 | 67.14 | 38850 | −0.451 | 20 | 10 | |
| 50 | Mufp3 v7 | 1–25 | 1–19 | 7.93 | 8699.60 | 15.74 | 67.56 | 41370 | −0.428 | 20 | 10 | |
| 51 | Mufp3 v8 | 1–27 | 1–22 | 9.19 | 8612.64 | 15.36 | 60.00 | 32890 | −0.406 | 20 | 10 | |
| 52 | Mufp3 v9 | 1–27 | 1–24 | 7.90 | 8453.68 | 15.74 | 70.00 | 42860 | −0.373 | 20 | 10 | |
| 53 | Mufp3 v10 | 1–25 | 1–22 | 9.30 | 8609.75 | 17.49 | 66.28 | 36900 | −0.110 | 20 | 10 | |
| 54 | Mufp3 v11 | 1–26 | 1–22 | 8.83 | 9034.05 | 20.9 | 64.62 | 41370 | −0.310 | 20 | 10 | |
| 55 | Mufp3 v12 | 1–27 | 1–19 | 7.90 | 8725.63 | 15.74 | 67.56 | 42860 | −0.404 | 20 | 10 | |
| 56 | Mytilus unguiculatus | Mufp3 v13 | 1–27 | 1–19 | 9.63 | 8625.82 | 14.03 | 64.62 | 19940 | −0.184 | 20 | 10 |
| 57 | Mufp3 v14 | 1–26 | 1–19 | 10.05 | 8196.27 | 25.11 | 54.17 | 38390 | −0.772 | 20 | 10 | |
| 58 | Mufp6 | 1–22 | 1–22 | 9.05 | 14175.06 | 38.31 | 39.18 | 36510 | −0.678 | 20 | 10 | |
| 59 | Mufp6 v1 | 1–25 | 1–25 | 9.13 | 14410.36 | 39.18 | 34.07 | 36510 | −0.694 | 20 | 10 | |
| 60 | Mufp6 v2 | 1–25 | 1–22 | 9.13 | 14455.30 | 38.70 | 34.88 | 39490 | −0.750 | 20 | 10 | |
| 61 | Mufp6 v3 | 1–25 | 1–22 | 9.07 | 14392.29 | 40.29 | 37.24 | 36510 | −0.669 | 20 | 10 | |
| 62 | Mufp6 v4 | 1–25 | 1–25 | 9.18 | 14386.30 | 40.58 | 31.15 | 38000 | −0.788 | 20 | 10 | |
| 63 | Mufp6 v5 | 1–25 | 1–25 | 9.12 | 14454.42 | 36.96 | 34.07 | 38000 | −0.727 | 20 | 10 | |
| 64 | Mufp6 v6 | 1–25 | 1–25 | 9.07 | 14538.45 | 33.80 | 27.72 | 36510 | −0.677 | 20 | 10 | |
| 65 | Mufp6 v7 | 1–25 | 1–25 | 9.16 | 11972.66 | 36.17 | 37.25 | 30300 | −0.639 | 20 | 10 | |
| 66 | Mufp6 v8 | 1–21 | 1–17 | 9.08 | 11396.92 | 40.19 | 39.18 | 31790 | −0.668 | 20 | 10 | |
| 67 | Mufp6 v9 | 1–24 | 1–16 | 8.88 | 11138.42 | 33.51 | 30.52 | 30300 | −0.938 | 20 | 10 | |
| 68 | Mizuhopecten yessoensis | Myfp1 v1 | ND | ND | 5.12 | 56961.06 | 23.62 | 6.97 | 8480 | −2.038 | 20 | 10 |
| 69 | Myfp1 v2 | ND | ND | 8.66 | 33579.64 | 62.60 | 56.48 | 118370 | −0.615 | 20 | 10 | |
| 70 | Perna canaliculus | Pcfp1 v1 | 1–20 | 1–16 | 9.92 | 51565.91 | −10.03 | 65.96 | 131995 | −0.637 | 20 | 10 |
| 71 | Pcfp1 v2 | 1–20 | 1–16 | 9.92 | 51078.31 | −9.97 | 65.90 | 130505 | −0.637 | 20 | 10 | |
| 72 | Pcfp1 v3 | 1–20 | 1–16 | 9.97 | 48252.05 | −10.61 | 66.83 | 127150 | −0.673 | 20 | 10 | |
| 73 | Pcfp1 v4 | 1–20 | 1–16 | 9.97 | 46301.65 | −10.38 | 66.60 | 121190 | −0.674 | 20 | 10 | |
| 74 | Perna viridis | Pvfp1 v1 | 1–20 | 1–20 | 11.20 | 63247.45 | 11.20 | 32.57 | 510930 | −0.800 | 20 | 10 |
| 75 | Pvfp1 v2 | 1–28 | 1–20 | 11.15 | 48042.87 | 15.66 | 33.67 | 362430 | −0.780 | 20 | 10 | |
| 76 | Pvfp3 | 1–23 | 1–23 | 5.93 | 7919.17 | 44.26 | 64.00 | 10595 | 0.260 | 20 | 10 | |
| 77 | Pvfp5 | 1–18 | 1–18 | 9.29 | 19695.09 | 24.06 | 33.75 | 39000 | −0.514 | 20 | 10 | |
| 78 | Pvfp6 | 1–19 | 1–17 | 6.48 | 13262.41 | 39.62 | 57.46 | 12920 | 0.102 | 20 | 10 | |
pI (Isoelectric point), Mw (Molecular weight), II (Instability index), AI (Aliphatic index), EC (Extinction coefficient) and GRAVY (Grand average of hydropathicity). ND: Not detected.