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. 2020 Feb 13;11:822. doi: 10.1038/s41467-020-14551-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Phase contrast images showing distinct morphologies of stromal and tumour cells. Scale bar, 200 µm. b Time-lapse imaging measuring confluency showing suppression of proliferation by 1 µM cisplatin and 100 nM paclitaxel. c Immunofluorescence images showing expression of PAX8 and Ki67. Scale bar, 50 µm. d Flow cytometry profiles quantitating the tumour markers EpCAM and CA125, and the stromal markers CD44 and CD105. Numbers represent percentage of cells in the quadrant. e Immunofluorescence images of Nutlin-3-treated cells (OCM.79) showing stabilisation of p53 in stromal cells but not tumour, and DNA sequence showing TP53 mutation in tumour cells (OCM.38a). Scale bar, 20 µm. Data in panels a and c are derived from analysis of OCM.79, while data in panels b and d are derived from analysis of OCMs 38a, and 66-5 respectively. Panels a, c and e are representative images from single experiments. Source data for panels b, c and d are provided as a Source Data file, including the gating/sorting strategy for panel d. See also Supplementary Figs. 1 and 2.