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. 2020 Feb 13;11:822. doi: 10.1038/s41467-020-14551-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Heat maps quantitating total chromosome count (T, range 40–>100) and individual chromosome counts (matrix, range 0–8), for OCMs 38b, 66–1 and 79, enriched for tetraploidy, rearranged chromosomes and whole-chromosome aneuploidy features respectively. Derivative chromosomes indicated by white. HCT116, a near-diploid, stable cell line with two derivative chromosomes, is shown for comparison. b M-FISH analysis of OCM.59. Heat map, exemplar chromosome spread and two exemplar M-FISH images. Heat map shows total chromosome count (T) and individual chromosome counts (matrix, range 0–6), quantitating recurring derivatives (A to M), unique derivatives (U), DNA fragments and micro-chromosomes (Micro), and other abnormal structures including dicentrics and ring chromosomes (Dic/Rg). The chromosome spread shows a micro (arrow) and dicentric (arrowhead) chromosome while the M-FISH images show whole chromosome aneuploidies, rearranged chromosomes and different derivatives. c Immunofluorescence images of cells stained to detect phospho-histone H3 (serine 10), Aurora A and the DNA (representative images from single experiment). Scale bar, 10 µm. d Quantitation of mitotic spindle poles in stromal cells, OCM tumour cells and nine established cell lines. Numbers outside the symbols indicate OCM culture while numbers inside the symbols indicate passage number. Orange arrows connect tumour samples from the same OCM culture analysed at different passages. Source data for panels a, b and d are provided as a Source Data file.