Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 14;5:13. doi: 10.1038/s41392-020-0111-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Western blotting and quantification analyses of the effect of MYH9 depletion on GSK3β, β-catenin, APC, and AXIN1 stability in HCCLM3 cells incubated with cycloheximide or MG132 at the indicated time points. b Western blotting and quantification analyses of the impact of HBX and MYH9 on GSK3β stability in HCCLM3 cells incubated with cycloheximide at the indicated time points. c Coimmunoprecipitation analyses were conducted to identify the function of HBX and MYH9 on the interplay between MYH9, TRAF6, ubiquitin, β-catenin, and GSK3β in HCCLM3 and PLC/PRF/5 cells incubated with MG132. d Coimmunoprecipitation analyses of the interaction between TRAF6, ubiquitin, and wild-type GSK3β or mutant GSK3β in HCCLM3 cells treated with MG132. e Western blot analyses were used to assess the impact of ubiquitin on GSK3β expression levels in ubiquitin-overexpressing HCCLM3 cells and the corresponding control cells.