Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 13;10:2569. doi: 10.1038/s41598-020-59541-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The long-term non-adjustive ADV-condition desynchronized SCN neurons. (A) Representative bioluminescence images of SCN slice culture from Per2^Luc mice under the indicated conditions for ~1 year. The animals were sampled at ZT12 on the second day after the shift. Hours indicate time after the last light onset. Scale = 200 µm. (B,C) Averaged bioluminescence traces from SCN slice cultures (mean ± SD, n = 3) and the FFT spectral analysis of the traces. Two-tailed Student’s t-test, *P < 0.05. Black and white bars show the animal’s previous LD conditions. (D) The detrended data of whole bioluminescence traces of the SCN slice culture from the indicated conditions. Orange boxes indicate the duration used for the single-cell level analysis. (E) ROIs for single-cell bioluminescence traces were represented in SCN images from each condition. LD, n = 14; ADV, n = 10. Scale = 250 µm. (F,G) The detrended traces at the single-cell level and the amplitude of the detrended traces. Two-tailed Student’s t-test, ****P < 0.0001.