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. 2020 Feb 13;10:2542. doi: 10.1038/s41598-020-59011-5

Figure 2.

Figure 2

Motor behavior of 6-OHDA lesioned D5 KO mice. Representative image of immunohistochemical analysis using anti-TH antibody of a coronal slice from 6-OHDA lesioned D5 KO mouse (A). Quantification derived from ImageJ density measurements is shown (E). The rotarod test was performed with the same animals before lesion (B), 3 weeks post lesion (C) and after 14 days of L-DOPA treatment (D) (N = 15 before and 12/10 after lesion for both WT/KO). The anti-akinetic response to L-DOPA was measured in the cylinder test (F). Statistical analysis was performed using two-way ANOVA: Effect of genotype: F(1,100) = 9.72, p = 0.002. Effect of time: F(4,100) = 4.79, p = 0.001. Post-hoc comparisons (Sidak) (*p < 0.05). At each trial days mice were subjected to 5 sessions spaced 20 minutes apart. For (F), the test was administered starting at 90 min post L-DOPA injections. Graphs show mean values +/− SEM. Statistical analysis was performed using 2-way ANOVA and Sidak’s multiple comparisons post-test (*P < 0.05).