Fig. 2. Verification of bap to identify barcode multiplets using 10× scATAC-seq data.

a Schematics of methodology to detect barcode multiplets whereby cellular nucleic acids are tagged by two different oligonucleotide sequences and later inferred from sequencing a scATAC-seq library from the same Tn5 insertions per fragment. b Schematic of mixing experiment. Two channels were combined and the resulting merged files were analyzed with bap. c–e Knee plots comparing the top 500,000 barcode pairs from c only channel 1, d only channel 2, and e between channels. The number of pairs calls is indicated by the number of points above the blue horizontal line (see Methods section).