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. 2020 Feb 13;11:868. doi: 10.1038/s41467-020-14708-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The top 12 strains from the initial screen (BC01–BC12), and the control strain (yGG066), were subject to reanalysis with n = 7 (n = 6 for the control strain) biologically independent samples using the same extraction and LC-MS workflow as in the initial screen. Data are presented as a box plot centred around the mean with bounds between the 25th and 75th percentile. Whiskers represent minima and maxima. b DNA from each of the top 12 strains was extracted and barcoded with 12 unique barcodes (BC01–BC12). The pooled DNA was then sequenced over 48 h. Throughput of bases (dark grey) and reads (light grey) was monitored to ensure no bias was introduced for a particular barcode. c Sequence analysis of the highest BA-producer, BC03 (left), indicates four SCRaMbLE events across three loci affecting ~20 kb of the chromosome. In contrast, BC11 (right) exhibits only a single non-coding deletion of 723 bp. Source data are provided as a Source Data file.