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. 2020 Feb 13;10:2588. doi: 10.1038/s41598-020-59518-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic analyses of SPFMV, SPVC, SPVG, SPLV, SPV2, and SPLCV isolates. (a) Maximum likelihood phylogenetic tree of genome sequences for 33 SPFMV isolates including six isolates from this study indicated by orange color. SPVC was used as an outgroup. (b) Maximum likelihood phylogenetic tree of genome sequences for 28 SPVC isolates including seven isolates in this study indicated by orange color. SPFMV was used as an outgroup. (c) Maximum likelihood phylogenetic tree of genome sequences for 12 SPVG isolates including two isolates in this study indicated by orange color. SPV2 was used as an outgroup. (d) Maximum likelihood phylogenetic tree of genome sequences for eight SPLV isolates including three isolates in this study indicated by orange color. Plum pox virus (PPV) was used as an outgroup. (e) Maximum likelihood phylogenetic tree of genome sequences for 14 SPV2 isolates including four isolates in this study indicated by orange color. SPVG was used as an outgroup. (f) Maximum likelihood phylogenetic tree of genome sequences for 14 SPLCV isolates including four isolates in this study indicated by orange color. SPGVaV was used as an outgroup. For phylogenetic analyses, we retrieved only complete genome sequences for each virus from GenBank based on a BLASTN search. We used not only complete viral genome sequences with respective accession numbers but also nearly complete viral genome sequences (Table S2) without accession numbers in this study. Accession number, isolate name, and virus name were described. Orange color indicates virus genomes obtained from this study. We used bootstrap replication values of 1,000, and bootstrap values over 70% are shown.