Figure 7.

Confirmation of 11 identified viruses infecting sweet potato by RT-PCR. (a) Position of amplicon on individual virus genome was indicated by gray bar with respective size of amplicon. The detailed information of primer pairs can be found in Table S3. (b) Agarose gel electrophoresis results by RT-PCR with newly designed primer pairs. Full-length gels of RT-PCR results can be found in Fig. S1 in the Supplementary Information. Actin gene of sweet potato was used as positive control. We used the same total RNA for both NGS and RT-PCR. Green color indicates RT-PCR primer pairs for two novel viruses, SPVE and SPVF, as well as a variant of SPVG.