Table 3.
Summary of de novo transcriptome assembly by Trinity.
| Index | Name of library | Total trinity transcripts | Percent GC | Contig N50 | Median contig length | Average contig | Total assembled bases |
|---|---|---|---|---|---|---|---|
| 1 | GJ | 73371 | 43.87 | 970 | 433 | 672.38 | 49333287 |
| 2 | GS | 132418 | 41.82 | 1507 | 535 | 902.08 | 119451072 |
| 3 | HN | 65866 | 44.43 | 838 | 405 | 612.85 | 40365766 |
| 4 | IC | 113660 | 42.62 | 1338 | 479 | 810.88 | 92164062 |
| 5 | IS | 176281 | 43.44 | 1047 | 434 | 694.65 | 122454002 |
| 6 | NS | 187838 | 44.19 | 982 | 420 | 665.55 | 125015489 |
| 7 | YA | 122883 | 42.01 | 1542 | 531 | 910.16 | 111843271 |
| 8 | YJ | 103809 | 42.96 | 1304 | 486 | 809.75 | 84058928 |
| 9 | YJ-B | 128922 | 42.16 | 1369 | 479 | 823.45 | 106161259 |
| 10 | YJ-H | 112891 | 42.46 | 1361 | 522 | 846.01 | 95507393 |
Raw sequence reads in each library were de novo assembled by Trinity. Statistical summaries of assembled contigs were obtained by Trinity toolkit using the “TrinityStats.pl” command.