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. 2020 Feb 13;11:867. doi: 10.1038/s41467-020-14525-4

Fig. 4. FMN supplementation facilitates protein folding.

Fig. 4

a Quantification of aggregate foci after heat shock for 90 min in wild type and fmn1 mutant yeast cells. Class1: one aggregate per cell (as highlighted by single GFP cluster); Class 2: two aggregates per cell; Class 3: ≥3 aggregates per cell. Bar graphs show fractions of Class 1, 2, and 3 cells for each strain. Above 500 cells were measured per sample with results shown as average values ± SD from six biological experiments. Scale = 2 µm. **p < 0.001. b Western blot of Aβ42 expression from stationary phase cultures, using the 6E10 Aβ specific antibody. GAPDH was used as the loading control. c Ten-fold serial dilutions of the control strain after 3 h of treatment with 5 or 10 mM L-azetidine-2-carboxylic acid (AZE). d Colony forming unit (CFU) measurement of control strain after 5 mM or 10 mM of AZE treatment as a measurement of viability (n = 3 ± SD). *p < 0.05. Source data are provided as a Source Data file.