Fig. 2. Validation and phenotypic grouping of CRISPR screen hits.

a Schematic depicting validation of the CRISPR screen by further KO of individual genes and stained by T5 mAb, Cholera enterotoxin subunit B (CTxB), and GS-II lectin. b Flow cytometry analysis of PIGS-KO HEK293 cells defective in each gene among 10 top-ranking protein-coding genes identified by our CRISPR screen. Cells were stained with T5 mAb, GS-II lectin and CTxB, respectively. See also Supplementary Fig. 1g–k and Supplementary Data 2 and 3. c Quantitative data of mean fluorescence intensity (MFI) from three independent experiments (mean ± SD, n = 3) based on stainings of T5 mAb (left), CTxB (middle), and GS-II (right). P values are from t test (unpaired and two-tailed) with comparisons to control (PIGS-KO). d Hierarchical clustering of glycan profiles analyzed by flow cytometry analyses, showing the effect (log₂ normalized MFI values) by each gene knockout based on staining of three probes. Source data are provided as a Source Data file.