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. 2020 Feb 13;11:860. doi: 10.1038/s41467-020-14678-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Left: HeLa cells stably expressing FLAG-6His tagged B3GALT4 were fixed and stained as described to detecting the FLAG tag (green) and a cis-Golgi marker (GM130; blue) and a TGN marker (TGN46; red). Scale bar, 10 μm. b Average localizations of the indicated Golgi proteins in HeLa cells (see Supplementary Fig. 6d) relative to GM130 (cis) and TGN46 (TGN) markers. Error bars indicate mean ± SD the number of measurements by linescan analysis for PGAP4 (41 images), B4GALNT1 (31 images), and B3GALT4 (29 images). c B3GALT4 colocalizes with the Golgi markers in HeLa cells. Left: Cells stably expressing FLAG-6His tagged B3GALT4 were fixed and stained for FLAG tag (green), as well as markers (magenta): Calnexin (ER), GM130 (cis-Golgi), B4GALT1 (Trans Golgi cisterna), and TGN46 (TGN). Scale bar, 10 μm. d Localization of B3GALT4 analyzed with 3D-SIM super-resolution microscopy. Left: a maximum intensity z-projection of B3GALT4 (green) and B4GALT1 or TGN46 (magenta), and a magnified region of Golgi in one of the cells. Right: ten magnified serial z-sections. For (a) and (b), right panel shows linescan analysis along a portion of the white line overlaying the image (left). Source data for (b) are provided as a Source Data file.