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. 2020 Feb 13;9(2):19. doi: 10.1038/s41389-020-0207-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The migration ability of iCCA cells was determined by wound-healing assays. The representative images were captured at 0 and 24 h. Bar = 300 μm. b and c Migration and invasion assays for the indicated cell lines after GLUT1 knockdown or overexpression. Bar = 100 μm. d Expression of MMP2, MMP7, and MMP9 was evaluated using western blot assays with the indicated processed cells and the corresponding control group. e MMP2 knockdown decreased the GLUT1-induced invasion of RBE cells. Bar = 100 μm. Data are means ± SD of three independent experiments. **P < 0.01; ***P < 0.001.