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. 2020 Feb 13;10:2558. doi: 10.1038/s41598-020-59517-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Screening of 29 NSAIDs on Mbnl1 mRNA expression. Undifferentiated C2C12 cells were cultured with 100 μM each of NSAIDs for 24 h. Expression levels of Mbnl1 and Gapdh were quantified by real-time RT-PCR. Expression level of Mbnl1 was normalized to that of Gapdh, and also to control cells with only DMSO. Mean and SD (n = 3 culture dishes) are indicated with individual values in red dots. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to DMSO alone by Student’s t-test with Bonferroni multiple comparison correction.