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. 2020 Feb 13;10:2558. doi: 10.1038/s41598-020-59517-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of knockdown of COX-1 or COX-2 on Tet1, Tet2, Tet3 and Dnmt3a mRNA expression in C2C12 cells. Real-time RT-PCR analysis was performed using undifferentiated C2C12 cells (a,b) or C2C12 cells differentiated for 5 days (c,d). The cells were treated with siRNA against COX-1 (siCOX1-a or siCOX1-b), COX-2 (siCOX2-a or siCOX2-b), both COX-1 and COX-2 (siCOX1a + 2a or siCOX1b + 2b), or control siRNA (siCont). Undifferentiated C2C12 cells were examined on 2 days after the transfection. Differentiated C2C12 cells were examined on differentiation day 5. Expression levels of Tet1, Tet2, Tet3, and Dnmt3a mRNA are normalized to that of Gapdh and also to siCont-treated cells. Mean and SD (n = 3 culture dishes) are indicated with individual values in red dots. *p < 0.05, **p < 0.01 and ***p < 0.001 compared to siCont by Student’s t-test with Bonferroni multiple comparison correction.