Table 3.
Mass spectrometry analysis of P. aeruginosa 3-oxo-C12-HSL and C4-HSL QS molecules release in response to SM23 treatment: time- and dose-dependence.
| 3-oxo-C12-HSL | C4-HSL | |||||
|---|---|---|---|---|---|---|
| Groups | 6 h | 12 h | 24 h | 6 h | 12 h | 24 h |
| Untreated | n.f. | 37.575.520,70 ± 2.315.838,91 | 201.150.062,20 ± 23.359.699,90 | n.f. | n.f. | 69.707.031,01 ± 1.143.025,45 |
| SM23 (0.780 μM) | n.f. | 23.645.421,84 ± 980.613,63* | 55.787.081,98 ± 5.098.512,17 | n.f. | n.f. | 56.319.203,07 ± 2.121.398,89 |
| SM23 (1.560 μM) | n.f. | 24.783.514,13 ± 339.890,28* | 51.160.160,28 ± 1.534.757,47 | n.f. | n.f. | 57.213.308,48 ± 1.431.287,96 |
| SM23 (3.125 μM) | n.f. | 26.816.303,02 ± 1.469.095,35* | 45.531.599,65 ± 4.653.861,29* | n.f. | n.f. | 48.215.432,52 ± 3.107.395,93* |
n.f., not found.
12 h: *p ≤ 0.05 SM23 treated vs untreated group according to ANOVA followed by Dunnett’s multiple comparisons test.
24 h: *p ≤ 0.05 SM23 treated vs untreated group according to Kruskall-Wallis followed by Dunnett’s multiple comparisons test.