Figure 4.

Magnolol increased the infiltration of IGF-1⁺ macrophages into muscle tissue. Wild type mice were given vehicle (con) or 10 mg/kg magnolol (mag) intraperitoneally 3 times for a week. Single cells were isolated from TA muscle and analyzed using flow cytometry. BrdU was injected 3 h before sacrifice for proliferation assay. (A) Representative dot plots of CD45 vs. IGF-1 within total single cells from TA muscle tissues. (B) Bar graph shows the percentage of CD45⁺IGF-1⁺ and CD45⁻ IGF-1⁺ populations in total cells. (C) Histograms showing the cell counts based on the F4/80 expression (white: isotype control; filled light gray: gated on CD45⁺IGF-1⁻; filled dark gray: gated on CD45⁺IGF-1⁺). (D) Percentages of F4/80⁺ macrophages in IGF-1⁺ or IGF-1⁻ cells among the CD45⁺ immune cells. (E,F) Percentages of IGF-1 expressing F4/80⁺ macrophages measured by gating on CD45⁺CD11b⁺ cells. (G) Representative FACS plots of CD11b⁺F4/80⁺ macrophages gated on CD45⁺ cells in muscle tissue and (H) bar graph showing the percentage of CD11b⁺F4/80⁺ cells in CD45⁺ cells. (I,J) Frequency of BrdU⁺ proliferating populations in CD11b⁺F4/80⁺ macrophages. All graphs are presented as the mean ± SEM (n = 4). *P < 0.05; **P < 0.01 vs. con based on the unpaired t test. The letters for no significance were not shown.