Figure 5.

Magnolol increased M2c-like macrophage subpopulations. Changes in immune cell subtypes by magnolol treatment were determined using flow cytometry in splenocytes from either control (con), magnolol (mag), cisplatin (cis), or cis+mag mice. (A) Gating strategy for analysis of macrophages and T cells subsets. Red dots display isotype controls and black dots show the stained cells with specific antibodies. (B) Representative dot plots of CD11b⁺F4/80⁺ macrophages, (C) CD8⁺ and CD4⁺ T cells. (D) Bar graphs showing mean% of CD11b⁺F4/80⁺ macrophages, (E) CD8⁺ T cells, and (F) CD4⁺ T cells in CD45⁺ total leukocytes in splenocytes. (G,H) Analysis of macrophage subtypes. (G) Representative dot plots identifying macrophages as CD163⁻CD206⁻ M1, CD163⁻CD206⁺ M2a, and CD163⁺CD206⁺ M2c gated on CD45⁺CD11b⁺F4/80⁺ cells. (H) Bar graphs showing mean% of M1, M2a, and M2c macrophages in CD11b⁺F4/80⁺ splenocytes. All data are presented as the mean ± SEM (n = 5). *P < 0.05; **P < 0.01; ***P < 0.001 vs. con, ^##P < 0.01; ^###P < 0.001 vs. mag, and ^$$P < 0.01 vs. cis based on the one-way ANOVA Tukey's test. The letters for no significance were not shown.