Figure 7.

Magnolol modulated the activation of bone marrow-derived macrophages in vitro. To assess the effect of magnolol on macrophage activation, C57 bone marrow cells were differentiated into M0 (M-CSF), M1 (M-CSF+LPS), or M2 (M-CSF+IL-4) macrophages and treated with magnolol. (A) Images showing the cell morphology of M0, M1, or M2 macrophages treated with (0, 0.1, 1, and 10) μM magnolol (Scale bar: 50 μm). (B–E) mRNA quantification of (B) iNOS, (C) CD163, (D) MMP-8, and (E) IGF-1 as assessed by qPCR. The images are representative of three independent experiments, and the quantification results were calculated by averaging across three separate experiments. *P < 0.05; **P < 0.01; ***P < 0.001 using two-way Bonferroni post-test.