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. 2020 Feb 13;10:2576. doi: 10.1038/s41598-020-59148-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Matrigel improved cell survival compared with the absorbable collagen sponge and POC/TCP. Cells were cultured in (A) absorbable sponge, (B) POC/TCP, or (C) Matrigel. (A,B) Cell survival was poor when cultured for 3 days. (C) Cell survival was high after 2 weeks. Scale bars = 400 µm. (D) Quantification of cell survival in the different materials. Cell survival in Matrigel was significantly higher than that in the other two materials (data are the mean ± SEM; *p < 0.05). (E) Laser confocal microscopy images showing that primary NSCs could differentiate into neurons in Matrigel (×400).