Figure 3.

Identification of positive Arabidopsis over-expressing lines. (a) Reverse transcriptase polymerase chain reaction analysis of SIMYB75 in the wild type (Col-0) and transgenic lines (#L1, #L2 and #L3).The Actin 2 gene was used as internal control. (b) Evaluation of Arabidopsis SiMYB75 over-expressing lines (#L1, #L2 and #L3) and wild type plants (Col-0) under mannitol (0/100 mM) and sodium chloride (NaCl) (0/100 mM) treatments at the germination stage. Percentage of germinated seeds with green cotyledons recorded after 7 days. For each experiment, about 30 plants/ lines were used. Data represent means ± SD of five measurements from one experiment and the experiment was repeated once more and similar results were obtained. Different letters above bar mean significant difference between each transgenic line and Col-0 at P ≤ 0.05.