FIGURE 2.

3D dMRI/XRPCT co-registration of the mouse brain and spinal cord. From the same subject, 3D dMRI and XRPCT image datasets were obtained. The native resolution of dMRI was 125 μm³ isotropic resolution (A,G), where XRPCT image spatial resolution was 6 μm (B,C,H). The scale bar is 500 μm in the brain and 200 μm in the spinal cord. To facilitate the co-registration, masking of both FA and XRPCT images (D,I) and down-sampling of XRPCT images to the image resolution of dMRI maps (E,J) were performed. In the case of the spinal cord, we performed the straightening of the whole spinal cord. In the background of panels (F,K), grayscale images represent FA map of the mouse brain, and yellow rendering in the brain and red in spinal cord show the XRPCT data. (L) Vascular segmentation of the co-registered spinal cord.