Table 1.

Comparison of serological and molecular tests of group without ocular toxoplasmosis (G1) with group with ocular toxoplasmosis (G2).

Groups	Serological tests ELISA/ELFA positive		Molecular tests cPCR (JW62/63)/cPCR (B22/23)/Nested PCR/qPCR positive
	IgM	IgG
G1 (185 samples)	6 (3.2%)/2 (1.1%)	121 (65.4%)/119 (64.3%)	1 (0.5%)/1 (0.5%)/3 (1.6%)/3 (1.6%)
G2 (164 samples)	10 (6.1%)/6 (3.7%)	158 (96.3%)/156 (95.1%)	3 (1.8%)/10 (6.1%)/4 (2.4%)/14 (8.5%)

Statistical analysis of IgG in G1. ELISA vs. ELFA: P-value = 0.913; 95% CI (0.875–1.181); IgM—ELISA vs. ELFA: P-value = 0.283; 95% CI (0.613–14.677); cPCR (JW62/63) vs. Nested PCR vs. cPCR (B22/23) vs. qPCR: P = 0.567; GL = 3; χ² = 2.022. Statistical analysis of IgG in G2. ELISA vs. ELFA: P-value = 0.785; 95% CI (0.967–1.06); IgM—ELISA vs. ELFA: P-value = 0.443; 95% CI (0.619–4.481); cPCR (JW62/63) vs. Nested PCR vs. cPCR (B22/23) vs. qPCR: P = 0.012; GL = 3; χ² = 10.936.

Statistical analysis of G1: cPCR (JW62/63) vs. Nested PCR vs. cPCR (B22/23) vs. qPCR: P = 0.567; GL = 3; χ² = 2.022. Statistical analysis of G2: cPCR (JW62/63) vs. Nested PCR vs. cPCR (B22/23) vs. qPCR: P = 0.012; GL = 3; χ² = 10.936.