Skip to main content
. 2020 Feb 7;10:3150. doi: 10.3389/fimmu.2019.03150

Figure 3.

Figure 3

The effects of IL-6 deficiency on macrophage infiltration and CCL2 expression in thrombus tissues. (A) Immunohistochemical analysis was performed using anti-F4/80 mAb at day 5 in venous thrombus samples from wild-type (WT) and Il6−/− mice (original magnification, ×400). Representative results from six independent experiments are shown here. (B) F4/80-positive macrophage numbers were determined as described in section Materials and Methods. All values represent the mean ± SEM (n = 6 animals). *p < 0.05, WT vs. Il6−/− (C) Intrathrombotic expression of Ccl2 mRNA after inferior vena cava (IVC) ligation was determined by real-time reverse transcription (RT)–PCR as described in section Materials and Methods. All values represent the mean ± SEM (n = 6 animals). **p < 0.01, WT vs. Il6−/−. (D) Immunohistochemical analyses of intrathrombotic CCL2. Representative results from six independent experiments are shown here. (E) Intrathrombotic CCL2-positive cell numbers were determined. All values represent the mean ± SEM. *p < 0.05, WT vs. Il6−/−.