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. 2020 Feb 7;11:174. doi: 10.3389/fmicb.2020.00174

FIGURE 7.

FIGURE 7

Real-time measurement of the cytotoxicity of BSN-37 and pexiganan against IPEC-J2 cells and Vero cells and the survival rates of IPEC-J2 cells and Vero cells incubated with 100 μg/ml BSN-37 and 100 μg/ml pexiganan for different times. (A,C) Real-time monitoring of the cytotoxicity of IPEC-J2 cells and Vero cells, respectively, treated with BSN-37 at a different concentrations (50, 100, 200, 400, and 800 μg/ml) and pexiganan at the concentration of 100 μg/ml for 60 h using the xCELLigence system. The black arrows indicate the time points of adding the peptides. The cells without antimicrobial peptide were used as control. (B,D) parallel comparisons of the survival rates of IPEC-J2 cells and Vero cells, respectively, treated with 100 μg/ml BSN-37 and 100 μg/ml pexiganan at specific four time points. Error bars represent means ± SEM, n = 3. *** and **** represents very significance and extremely significance, respectively. (p < 0.001 and p < 0.0001 by Two-way ANOVA).