Figure 5.

miR-433-3p Targets ANXA2

(A) Predicted binding sites of miR-433-3p on ANXA2-3′ UTR. (B) Luciferase activity detection of ANXA2-WT/ANXA2-MUT after miR-433-3p mimic transfection. (C) The mRNA expression of ANXA2 after miR-433-3p mimic or miR-433-3p inhibitor transfection determined by qRT-PCR. (D) The protein expression of ANXA2 after miR-433-3p mimic or inhibitor transfection determined by western blot assay. *p < 0.05 versus the NC mimic group; # p < 0.05 versus the NC inhibitor group. Comparisons between two groups were analyzed using two-tailed independent t test; the comparison among multiple groups was analyzed by one-way analysis of variance with Tukey’s post hoc test. miR-433-3p, microRNA-433-3p; ANXA2, Annexin A2; UTR, untranslated region; NC, negative control.