Immunohistochemical Staining Confirmed the Cells Surviving and Expressing LEF1 from the Engrafted MI + LEF1/hUCB-MSCs
(A) LEF1 was detected only from MI + LEF1/hUCB-MSCs. No rat heart cells and hUCB-MSCs expressed LEF1. Scale bars, 100 μm. (B) LEF1-positive cells in the heart tissue were quantitatively measured (n = 5). (C) No lamin signal was detected in the MI-alone group. A thin layer was found in the group of MI + hUCB-MSCs, and a thicker layer was stained from MI + LEF1/hUCB-MSC-engrafted MI. Scale bars, 100 μm. (D) Human cell engraftment was quantitatively measured (n = 5). **p < 0.01.