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. 2020 Feb 7;11:18. doi: 10.3389/fpls.2020.00018

Figure 4.

Figure 4

Expression pattern and subcellular localization of MtABCG59. (A) GUS staining of 2-week-old transgenic Medicago truncatula expressing ProMtABCG59:GUS and quantitative PCR expression analysis of MtABCG59 in different M. truncatula organs (three-month-old plants) revealed the MtABCG59 transcript accumulation exclusively in the roots and nodules. The transcript levels were normalized to the actin gene from M. truncatula. Data represent the mean ± SD of three independent biological experiments and three technical repeats. (B) X-Gluc staining of ProMtABCG59:GUS reporter line in root cortex (upper panel) and in root meristem (middle panel), X-Gluc staining of root cross-sections (bottom panel). (C) Fluorescence signal of ProMtABCG59:NLS-GFP reporter line in root cortex (upper panel) and in root meristem (bottom panel). (D) Arabidopsis mesophyll protoplast expressing the fusion gene Pro35S:GFP-MtABCG59. The GFP signal was observed in the plasma membrane (left panel). Control Arabidopsis protoplast expressing free cytoplasmic GFP (right panel). The red color represents chlorophyll autofluorescence.