TABLE 2.

Proportional variance (η²) values for age, genotype and sex as assessed by three-way ANOVA.

Variable	Age	Genotype	Sex	Age × Genotype	Age × Sex	Genotype × Sex
Physiological parameters
Body weight (g)	0.4993	0.0011	0.0732	0.0005	0.0010	0.0041
HW:TL, mg/mm	0.2825	0.0233	0.1745	0.0332	0.0258	ns
HR, beats/min	0.0040	0.0105	0.0020	ns	0.0191	0.0021
Protein expression
pAKT/AKT	0.5828	0.0029	0.0355	0.0253	0.0046	0.0022
Cardiac sEH	0.0185	0.8821	0.0092	0.0185	0.0080	0.0092
Cardiac mEH	0.5598	0.0437	0.0617	0.0352	0.0326	0.0157
Renal sEH	0.0003	0.6771	0.1524	0.0003	0.0001	0.1524
Renal mEH	0.3350	0.0178	0.0036	0.0022	0.0043	0.0312
Cardiac Sirt-3	0.0012	0.0060	0.0096	0.0457	0.0160	0.0108
Cardiac AcMnSOD	0.4116	0.3121	0.0004	0.0023	0.0007	0.0029
Renal Sirt-3	0.0133	0.0565	0.0293	0.0023	0.0491	0.0304
Renal AcMnSOD	0.1294	0.0681	0.0015	0.0049	0.0478	0.0231
Enzymatic activity
Cardiac Sirt-3, U ⋅ μg–1	0.2208	0.0643	0.0598	0.0562	ns	0.0209
SOD, U ⋅ mg–1	0.3586	0.1958	0.1017	0.0708	0.0123	0.0033
Protein Carbonylation, nmol ⋅ mg–1	0.0800	ns	0.0337	0.0365	0.2499	0.1725
Citrate Synthase, nmol ⋅ min–1mg–1	0.1827	0.1680	0.0481	0.0001	0.0063	0.0009
2D ECHO: Wall measurements
EF,%	0.2549	0.0001	0.0420	0.0055	0.0121	0.0016
FS,%	0.2476	ns	0.0374	0.0071	0.0081	0.0015
LVEDV, μl	0.1663	0.0119	0.1092	0.0016	0.0045	0.0155
LVESV, μl	0.2293	0.0055	0.0846	0.0023	0.0183	0.0010
CO, ml/min	0.0575	0.0078	0.0460	0.0004	0.0042	0.0481
SV, μl	0.0492	0.0163	0.0910	0.0004	0.0012	0.0449
Corrected LV mass, mg	0.3063	0.0119	0.0928	0.0192	0.0078	0.0186
LA, mm	0.3451	0.0011	0.0064	0.0005	0.0041	0.0032
IVS-diastole, mm	0.1061	0.0000	0.0057	0.0521	0.0278	0.0245
IVS-systole, mm	0.0800	0.0122	0.0043	0.0099	0.0000	0.0281
LVPW-diastole, mm	0.1490	0.0004	0.0493	0.0130	0.0006	0.0071
LVPW-systole, mm	0.0249	0.0109	0.0283	0.0101	0.0184	0.0140
LVID-diastole, mm	0.1769	0.0099	0.0866	0.0012	0.0059	0.0108
LVID-systole, mm	0.2497	0.0034	0.0737	0.0033	0.0112	0.0013
2D ECHO: Doppler imaging
IVRT, ms	0.0011	0.0299	0.0013	0.0030	ns	0.0055
IVCT, ms	0.0140	0.0176	0.0181	0.0011	0.0101	0.0004
AET, ms	0.2371	0.0081	0.0050	0.0035	0.0241	0.0015
Tei index	0.1613	0.0214	0.0179	0.0017	0.0020	0.0001
E/A	0.1864	0.0246	0.0082	0.0103	0.0042	0.0117
E′/A′	0.0237	0.0002	0.0207	0.0003	0.0000	0.0066
E′	0.0006	0.0004	0.0014	0.0030	0.0394	0.0095
E/E′	0.0494	0.0284	0.0201	0.0241	0.0148	0.0054

Variances are reported as a fraction of 1. Bolded values represent variances that reach a statistically significant contribution of the total variability, P < 0.05.