TABLE 3.

P-values from three-way ANOVA statistical analysis of data-set variability.

Variable	Age	Genotype	Sex	Age × Genotype	Age × Sex	Genotype × Sex
Physiological parameters
Body weight (g)	<0.0001	0.4404	<0.0001	0.5936	0.4583	0.1366
HW:TL, mg/mm	<0.0001	0.0474	<0.0001	0.0190	0.0374	0.9715
HR, beats/min	0.4141	0.1860	0.5623	0.9422	0.0755	0.5523
Protein expression
pAKT/AKT	<0.0001	0.5743	0.0556	0.1034	0.4802	0.6218
Cardiac sEH	0.0043	<0.0001	0.0371	0.0043	0.0513	0.0371
Cardiac mEH	<0.0001	0.0771	0.0384	0.1103	0.1235	0.2783
Renal sEH	0.6159	<0.0001	<0.0001	0.6159	0.7943	<0.0001
Renal mEH	0.0075	0.4902	0.7553	0.8066	0.7332	0.3642
Cardiac Sirt-3	0.8365	0.6480	0.5653	0.2143	0.4585	0.5423
Cardiac AcMnSOD	<0.0001	<0.0001	0.8027	0.5654	0.7577	0.5196
Renal Sirt-3	0.6153	0.3061	0.4578	0.8346	0.3391	0.4496
Renal AcMnSOD	0.1080	0.2347	0.8553	0.7437	0.3160	0.4819
Enzymatic activity
Cardiac Sirt-3, U ⋅μg–1	<0.0001	0.0187	0.0231	0.0275	0.9616	0.1730
SOD, U ⋅ mg–1	<0.0001	0.0013	0.0140	0.0360	0.3605	0.6308
Protein carbonylation, nmol ⋅ mg–1	0.0496	0.9944	0.1923	0.1753	0.0012	0.0056
Citrate synthase, nmol ⋅ min–1mg–1	0.0133	0.0170	0.1819	0.9639	0.6240	0.8539
2D ECHO: Wall measurements
EF,%	<0.0001	0.8915	0.0017	0.2520	0.0885	0.5388
FS,%	<0.0001	0.9607	0.0034	0.1970	0.1686	0.5572
LVEDV, μl	<0.0001	0.0836	<0.0001	0.5300	0.2893	0.0491
LVESV, μl	<0.0001	0.2324	<0.0001	0.4358	0.0295	0.6135
CO, ml/min	0.0010	0.2195	0.0031	0.7773	0.3677	0.0025
SV, μl	0.0017	0.0674	<0.0001	0.7803	0.6119	0.0026
Corrected LV mass, mg	<0.0001	0.0379	<0.0001	0.0087	0.0919	0.0098
LA, mm	<0.0001	0.6172	0.2364	0.7435	0.3450	0.4060
IVS-diastole, mm	<0.0001	0.9723	0.3089	0.0024	0.0252	0.0357
IVS-systole, mm	0.0001	0.1253	0.3639	0.1670	0.9575	0.0208
LVPW-diastole, mm	<0.0001	0.7640	0.0014	0.0969	0.7176	0.2189
LVPW-systole, mm	0.0351	0.1611	0.0250	0.1785	0.0694	0.1128
LVID-diastole, mm	<0.0001	0.1205	<0.0001	0.5948	0.2308	0.1058
LVID-systole, mm	<0.0001	0.3423	<0.0001	0.3524	0.0875	0.5627
2D ECHO: Doppler imaging
IVRT, ms	0.6663	0.0276	0.6412	0.4789	0.9306	0.3423
IVCT, ms	0.1210	0.0825	0.0785	0.6611	0.1865	0.8040
AET, ms	<0.0001	0.1804	0.2914	0.3758	0.0217	0.5603
Tei index	<0.0001	0.0379	0.0573	0.5511	0.5252	0.9008
E/A	<0.0001	0.0233	0.1893	0.1401	0.3476	0.1164
E′/A′	0.0540	0.8518	0.0713	0.8288	0.9445	0.3068
E′	0.7670	0.8147	0.6478	0.4998	0.0155	0.2318
E/E′	0.0045	0.0301	0.0670	0.0455	0.1154	0.3413

Bolded values represent variables that elicit statistically significant effects on the parameter in question, P < 0.05.