Table 5.

Comparison of normalization methods used, including execution time (rounded to nearest five seconds), visualization, and classification rate across all data sets.

Category	BASiCS	GRM	Linnorm	SAMstrt	SCnorm	scran	Simple Norm
Mouse Embryonic Data (sec)	230	35	<5	<5	760	<5	<5
Mouse Lung Data (sec)	510	60	<5	5	1180	<5	<5
Mouse Embryonic Sim Data (sec)	110	15	<5	<5	110	<5	<5
Human Embryonic Data (sec)	_	_	<5	_	370	<5	<5
Classification (Spike-In Genes)	***	*	**	*	***	**	**
Visualization (Spike-In Genes)	**	*	**	*	**	**	**
Classification (Non-Spike-In)	_	_	*	_	**	**	**
Visualization (Non-Spike-In)	_	_	*	_	**	*	**

A ‘***' indicates that the method showed exemplary performance overall, a ‘**' indicates satisfactory performance overall, and a ‘*' indicates some shortcoming in performance compared to other methods. ‘_' indicates that the method is not applicable for this type of data. Two variants exist for the Mouse Embryonic Sim Data, but they had negligible differences in execution time.